ETTAN SPOT PICKER PDF

Enjoy Free Shipping on most orders placed online — Terms Apply. To leave a review Register or Log In. Selected purification special offers available for a limited time only — save today! Allows quick and accurate protein spot picking from nonbacked 2-D gels, increasing the versatility of Ettan Spot Picker.

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Automatically picks selected protein spots from stained or destained gels, with greater than Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation. Increase the aspiration volume. Check the O-ring of the picker head for damage.

Check all tubing for leaks and air bubbles. Gel plug is situated in a nearby well. The microplate Z-height has been too high so the dispense flow has splashed it into a nearby well. Gel plug is in rinse station due to adsorption to the outer side of the picker head during dispensing. The dispensing height has been set too low. Increase the microplate Z-height. Clean the outer side of the picker head to reduce liquid adsorption. The dispensing volume has been set too low.

Increase the dispensing volume. Be sure to have a distinct circular picture of the marker both in the camera and marker detection view. Damaged reference markers and impurities in the gel image can result in incorrect detections. Also, reflections, shadows and incorrect set contrasts, thresholds and camera aperture can result in similar errors.

This will result in imprecise picking. Power failure routine In the event of a power failure, the experiment is interrupted in an undefined state. The data for gel plugs picked up to the power interruption is saved. When power returns, the following will happen: 1. The picker head and the syringe pump dilutor are homed. The PC starts and displays the log-in dialogue. Check that the Spot Picker is connected to a wall socket and that the mains switch is in the ON 1 position.

Gel stored in ethanol and acetic acid has a tendency to crack. This will not affect the picking result, if not too many spots are picked from the same area. Prepare according to recommendations in User Manual. Homing failed for the X and Yaxes. The cause may be that the instrument has collided while moving diagonally. Homing failed for the Y-axis. For details, see the User Manual. Homing failed for the X-axis.

Ettan Spot Picker Automatically picks selected protein spots from stained or destained gels, with greater than Please change the country on your profile in order to switch to another country store. Edit Profile. Product Support. In this section. Spare parts. Spare parts not indicated in the view. Something went wrong - we were not able to load your agreed MSA pricing.

Please try refreshing the page. Spare parts for Ettan Spot Picker. Select a symptom: Gel plugs not removed from gel. Possible cause Suggested remedy The set jazz amplitude is too low.

Increase the jazz value stepwise with 0. Inefficient aspiration. Bind-Silane treatment was too effective. Reduce the concentration of Bind-Silane. The picker head does not reach the gel. The picker head is worn-out. Replace the picker head.

A position, close to the current gel plug's, has previously been picked. Change me. Gel plugs are picked but deformed or not complete.

Possible cause Suggested remedy Only the upper part is picked due to the picker head not reaching the gel backing. Adjust the picker head versus gel Z-position. The inner side of the picker head is dirty. Clean the picker head both inner and outer sides. Gel from spot has been picked but is not found in microplate well. Possible cause Suggested remedy Gel plug is situated in a nearby well.

Decrease the microplate Z-height. Gel plug is lost during transfer from gel to microplate. Very imprecise picking results. Possible cause Suggested remedy The identities of the two reference markers have been mixed up. Confirm that the first detected marker is the correct one on the gel image.

Picking position errors. Possible cause Suggested remedy Camera is not correctly calibrated. Check that the calibration has been performed according to the Instrument Handbook Incorrect detection of the reference markers. Uncorrected ImageScanner image. Scan according to User manual and use the correction option when the pick list is imported. There is no calibration file for the used scanner. Contact GE Healthcares service representative for calibration of the scanner. The gel image has too low resolution.

Scan the gel with higher resolution, for example dpi. Incorrect reference marker detection. Possible cause Suggested remedy Damaged reference markers and impurities in the gel image can result in incorrect detections.

Picking errors for oval and irregular shaped spots. Possible cause Suggested remedy The centre of the detected spot in the 2D-software differs from the preferred picking position. Detect these types of spots manually. For example with the Grow Peaks tool or the Pen tool. Issues related to camera calibration procedure. Possible cause Suggested remedy Calibration foot is not visible in the camera view window although it is underneath the camera Adjust the aperture until the calibration foot is visible Calibration foot is visible in the camera view window but not visible in the detection window Adjust the Contrast and Threshold sliding bars until an image is visible in the detection window Calibration foot is not placed on the tape Decrease the Z-coordinate to make the picker head move closer to the tray.

Power failure. Possible cause Suggested remedy Power failure routine In the event of a power failure, the experiment is interrupted in an undefined state. No movement from the Spot Picker following command sent from the PC. Possible cause Suggested remedy Instrument not plugged in or mains switch turned off Check that the Spot Picker is connected to a wall socket and that the mains switch is in the ON 1 position The Stop button has been pressed Press the Start button on the Spot Picker RS cable not properly connected Check RS cable and ensure that it is plugged into the correct port.

Light not coming on. Possible cause Suggested remedy Power supply from 24 V output disconnected Check power cable from light to 24 V power supply Internal fuse has blow Please contact GE Healthcares service representative. Reference detection unstable. Possible cause Suggested remedy Contrast not properly set Adjust the aperture setting on the camera. No picture from the camera. The gel cracks and become damaged close to the picked gel plug.

Possible cause Suggested remedy Gel stored in ethanol and acetic acid has a tendency to crack. Storage of the gel in double distilled water reduces the risk of cracking. The gel loosens from the glass plate in a specific area during picking. Possible cause Suggested remedy Too many gel plugs have been picked from a smaller area.

The gel loosens from the glass plate. Possible cause Suggested remedy Inefficient Bind-Silane treatment. Scratches on the gel glass plates after picking.

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GE Healthcare Ettan Spot Picker

Don't have a profile? Additional Details : Weight : 6, Product Code. This item is currently unavailable or has been discontinued. View the product page for possible alternatives. Compatible with common staining methods such as silver, Coomassie Blue, fluorescence, and labeling with Ettan DIGE Fluor dyes Gel frame — a nonbacked gel slab is attached to a temporary cellophane backing, supporting reference markers and making them highly percise Improves nonbacked gel handling during scanning, and glass lid protects the moist gel. No offer available.

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Ettan Spot Picker Nonbacked Gel Kit

The following illustrations and descriptions refer to the instrument model and are drawn from brochures. They do not represent the scope of delivery. Please refer to the text of the offer for the exact scope of delivery. Technical Information. Ettan Spot Picker performs accurate and reliable picking of gel electrophoresis spots. A protein picklist is generated , either automatically using predefined selection criteria , or manually. The protein picklist is transferred to Ettan Spot Picker , which then automatically picks the protein spots according to the list.

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